Simultaneous determination of myocardial creatine phosphate and adenine nucleotides by reversed-phase HPLC
โ Scribed by Radhika Sanduja; G. A. S. Ansari; Paul J. Boor
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 231 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0269-3879
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๐ SIMILAR VOLUMES
A sensitive and specific assay for measurement of adenine nucleotides and adenosine by paired-ion high-performance liquid chromatography is described. The I,N6-ethenoderivatives of ATP (c-ATP), ADP (+ADP), AMP (c-AMP). and adenosine (c-Ado), formed by reaction with chloroacetaldehyde at 37ยฐC. were s
The developed method of ion-pair reverse-phase high-pressure liquid chromatography (hplc) enables one to separate adenine nucleotides in cardiac tissue extract within 10 min with recovery of about 98% and lowest detection limit of 10m6 M. Separation of creatine and phosphocreatine is performed by io