Simultaneous determination of ginsenosides and saikosaponins by high-performance liquid chromatography

✍ Scribed by Hideko Kanazawa; Yoshiko Nagata; Yoshikazu Matsushima; Masashi Tomoda; Nobuharu Takai

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 311 KB
• Volume: 507
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)84210-2

✦ Synopsis

Octadecyl porous glass was used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides and saikosaponins (saponins of ginseng and bupleurum root, respectively) were analysed with detection at 203 nm. A well resolved chromatogram of ginsenoside Rbl, Rc, Rbz and Rd and saikosaponin a, bz and c was obtained with acetonitrile-water (25.5~74.5) as the mobile phase. The whole separation was achieved in 20 min with a flow-rate of 1.5 ml/min. Calibration graphs for ginsenoside Rbi, Rc, Rbz and Rd and saikosaponin a and c were linear up to 5 pg. Rapid and accurate simultaneous determinations of the saponins are possible by the described method.