Simultaneous determination of creatinine and uric acid in human plasma and urine by micellar electrokinetic chromatography

✍ Scribed by Miyake, Mamoru ;Shibukawa, Akimasa ;Nakagawa, Terumichi

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 373 KB
• Volume: 14
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240140309

✦ Synopsis

Abstract

High performance capillary electrophoresis using a buffer solution containing micelles of ionic surfactant (e.g. sodium dodecyl sulfate), called micellar electrokinetic chromatography, has been applied to the separation and simultaneous determination of creatinine and uric acid in human plasma and urine. The sample was introduced into the capillary by siphoning an appropriate volume of untreated plasma or urine spiked with an internal standard (antipyrine). Creatinine, uric acid, and antipyrine were separated mutually, and from other endogeneous components within 18 min. The calibration plots showed good linearity (correlation coefficient > 0.999) over the concentration range needed for clinical analysis. Standard addition tests indicated that the recoveries of creatinine and uric acid from urine samples ranged, respectively, from 97 % to 106 % and 97.4 % to 108 % with a coefficient of variation (C.V.) of 3.3 % (n = 5), and that those from plasma samples ranged, respectively, from 100 % to 112 % and 101 % to 107 % with a C.V. of 4.7 % (n = 5). The results were in agreement with those obtained by conventional methods.