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Simultaneous determination of 1H–1H and 1H–13C residual dipolar couplings in a chiral liquid crystal solvent using a natural abundance HSQC experiment

✍ Scribed by Vasilios M. Marathias; Igor Goljer; Alvin C. Bach II

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
186 KB
Volume
43
Category
Article
ISSN
0749-1581

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✦ Synopsis

Abstract

A high‐resolution, phase‐sensitive, natural abundance F~2~‐coupled ^1^H–^13^C HSQC (F2HSQC) NMR experiment was developed to measure simultaneously both ^n^D~HH~ and ^1^D~CH~ residual dipolar couplings (RDCs) of small molecules present in a chiral polypeptide liquid crystal solvent system composed of poly‐γ‐benzyl‐L‐glutamate (PBLG) in CDCl~3~. Because this is an indirect‐detection NMR experiment, the relatively small amount of sample (7.5 mg in this study) and short acquisition times (5 h) that are required make this HSQC experiment well suited for samples that are either limited in solubility or in quantity or require short analysis times. The F2HSQC experiment can be performed without any specialized equipment or sample modification and can enhance our ability to measure RDCs accurately and rapidly in polypeptide liquid crystal solvents. Copyright © 2005 John Wiley & Sons, Ltd.

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