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Simultaneous detection of kinase and phosphatase activities of polynucleotide kinase using molecular beacon probes

✍ Scribed by Ma, Changbei; Fang, Hefei; Wang, Kemin; Xia, Kun; Chen, Hanchun; He, Hailun; Zeng, Weimin


Book ID
123520977
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
535 KB
Volume
443
Category
Article
ISSN
0003-2697

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We report a simple method that permits simultaneous detection of multiple protein kinase activities using postnuclear supernatant of \(\mathbf{v}-8\) src transformed NIH3T3 cells. A supernatant is incubated with activators of protein kinases and \(\left[\gamma-{ }^{32} \mathbf{P}\right] \mathrm{ATP}