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Highly sensitive detection of alkaline phosphatase using molecular beacon probes based on enzymatic polymerization

✍ Scribed by Changbei Ma


Book ID
116736151
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
277 KB
Volume
26
Category
Article
ISSN
0890-8508

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A novel strategy is described for highly sensitive DNA detection and point mutation identification based on the combination of reverse molecular beacon with DNA ligase. A 5'-phosphoryl and 3'-ferrocene terminated DNA sequence is used as detection probe, which may be ligated to capture DNA immobilize