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Simultaneous detection of DNA strand breaks and unscheduled DNA synthesis in mutagen-treated human lymphocytes in the absence of hydroxyurea

✍ Scribed by M. Holmberg; M. Lagerberg; B. Niejahr; L. Rödin


Book ID
113193491
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
565 KB
Volume
202
Category
Article
ISSN
0027-5107

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A modified assay for the detection of DNA single-strand breaks (SSBs) in human mononucleated white blood cells (MWBCs) based on the nick translation (NT) reaction was developed and combined with the test for unscheduled DNA synthesis (UDS). Both assays were performed on disposable 96-well filtration