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Simultaneous binding of a polyamide dimer and an oligonucleotide in the minor and major grooves of DNA

✍ Scribed by Michelle E. Parks; Peter B. Dervan

Publisher: Elsevier Science
Year: 1996
Tongue: English
Weight: 645 KB
Volume: 4
Category: Article
ISSN: 0968-0896
DOI: 10.1016/0968-0896(96)00089-2

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✦ Synopsis

The effect of the polyamide ImPyPy-Dp (Im = N-methylimidazole-2-carboxamide, Py = N-methylpyrrole-2-carboxamide, and Dp = dimethylaminopropylamide), which binds as an antiparallel dimer in the Watson-Crick minor groove, on pyrimidine. purine.pyrimidine triple helix stability was investigated. A DNA restriction fragment was designed which contained two triple helix sites, one which overlapped a minor groove ligand site (proximal), and a control site 13 base pairs away (distal). Using quantitative DNase I footprint titration experiments the equilibrium association constant of oligonucleotide 5'-TTTTTm5CTTTm5CTTTm5CT-3' (1) to each site was measured in the absence and presence of the polyamide dimer. Our data indicate that triple helix formation is compatible with a polyamide dimer binding in the minor groove of DNA at an overlapping site. No cooperative effect of the polyamide dimer on the equilibrium association constant of oligonucleotide 1 was observed.

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