Water and monovalent ions in the minor groove of B-DNA oligonucleotides as seen by NMR

✍ Scribed by Bertil Halle; Vladimir P. Denisov

• Publisher: Wiley (John Wiley & Sons)
• Year: 1998
• Tongue: English
• Weight: 363 KB
• Volume: 48
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/(sici)1097-0282(1998)48:4<210::aid-bip3>3.0.co;2-y

✦ Synopsis

During the past 8 years, two complementary nmr techniques-magnetic relaxation dispersion and nuclear Overhauser effect spectroscopy-have been applied extensively to the study of water and monovalent ions in the minor groove of B-DNA oligonucleotides in solution. In this review, the possibilities and limitations of the two methods are outlined, with emphasis on the interpretational steps whereby molecular-level information is extracted from the primary data. The results on sequence-dependent hydration and ion-DNA interactions obtained so far by these methods is summarized and critically assessed. The nmr results are also compared with structural data from x-ray crystallography.