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Simultaneous assay of serum vitamin A and vitamin E by high performance liquid chromatography using time-switched UV and fluorimetric detectors

โœ Scribed by Russell, M. J. ;Thomas, B. S. ;Wellock, E.

Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
327 KB
Volume
9
Category
Article
ISSN
0935-6304

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โœฆ Synopsis

An HPLC method utilizing a UV and a fluorimetricdetector linked in series is described. By use of a simple integrator-controlled time-switched relay, analysis of serum vitamins A and E is accomplished on the same chromatogram and at optimum sensitivity for each detector. A single internal standard (retinyl acetate) monitored only by the UV detector permits measurement of both vitamins over a wide linear range. Precision of the assays is satisfactory, both on a within-day and on a day-to-day basis. Recoveries of both vitamins are virtually 1000/0 whilst sensitivity is 2 pg/L (retinol) and 0.05 mg/L (a-tocopherol).

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