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Simultaneous analysis of monosaccharides and oligosaccharides by high-performance liquid chromatography with postcolumn fluorescence derivatization

✍ Scribed by Hirotaka Kakita; Hiroshi Kamishima; Katsuo Komiya; Yoshio Kato


Book ID
108339024
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
406 KB
Volume
961
Category
Article
ISSN
1873-3778

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A method is described for the detection of glutathione (GSH) and glutathione disulfide (GSSG) based on a HPLC postcolumn reaction with ortho-phthalaldehyde (OPT) at pH 12 followed by fluorescence detection. Although similar methods have been reported, the high pH of the postcolumn reaction adds cons