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Simultaneous analysis of hydrochlorothiazide, triamterene and reserpine in rat plasma by high performance liquid chromatography and tandem solid-phase extraction

✍ Scribed by Hang Li; Junting He; Qin Liu; Zhaohui Huo; Si Liang; Yong Liang

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
145 KB
Volume
34
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

A tandem solid‐phase extraction method (SPE) of connecting two different cartridges (C~18~ and MCX) in series was developed as the extraction procedure in this article, which provided better extraction yields (>86%) for all analytes and more appropriate sample purification from endogenous interference materials compared with a single cartridge. Analyte separation was achieved on a C~18~ reversed‐phase column at the wavelength of 265 nm by high‐performance liquid chromatography (HPLC). The method was validated in terms of extraction yield, precision and accuracy. These assays gave mean accuracy values higher than 89% with RSD values that were always less than 3.8%. The method has been successfully applied to plasma samples from rats after oral administration of target compounds.

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