Determination of hyperoside and isoquercitrin in rat plasma by membrane-protected micro-solid-phase extraction with high-performance liquid chromatography

✍ Scribed by Xiao-Ying Yin; Yong-Ming Luo; Jian-Jiang Fu; You-Quan Zhong; Qing-Shan Liu

• Publisher: John Wiley and Sons
• Year: 2012
• Tongue: English
• Weight: 325 KB
• Volume: 35
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.201100867

✦ Synopsis

Abstract

A novel method, micro‐solid‐phase extraction based on membrane‐protected molecularly imprinted polymer, was developed to extract hyperoside and isoquercitrin in rat plasma. Synthesized hyperoside MIPs were packed in a porous polyether sulfone membrane envelope to perform extraction. The parameters sorbent materials, membrane types, extraction time and desorption conditions were optimized for micro‐solid‐phase extraction. Under the optimal conditions, correlation coefficients, 0.998 and 0.999, were obtained for hyperoside and isoquercitrin, respectively, with the linear range between 1 and 120 μg/mL. The absolute extraction recoveries from 84.5 to 89.3% were found. The method detection limits of hyperoside and isoquercitrin were 0.24 and 0.22 μg/mL, respectively. Compared with traditional methods, solid‐phase extraction, liquid–liquid extraction and protein precipitation, the developed method was simple, highly efficient for extraction, environmentally friendly, and particularly suitable for complex biological samples.