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Simultaneous analysis of cell surface antigens and DNA content by flow cytometry

✍ Scribed by D. J. HOWE; J. A. HOPKINS; S. A. N. JOHNSON; M. J. PHILLIPS

Book ID
114716976
Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
309 KB
Volume
15
Category
Article
ISSN
1751-5521

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✍ Jan F. Keij; Carolyn Bell-Prince; John A. Steinkamp πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 106 KB πŸ‘ 2 views

Background: Analysis of the DNA cell cycle and glutathione content cannot be performed on viable cells, because the fluorescence emissions of the DNA-specific probe Hoechst 33342 and the glutathione-specific probe monobromobimane overlap completely. We decided to explore whether the emissions could