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Simultaneous analysis of cell surface antigens and cell morphology using monoclonal antibodies conjugated to fluorescent microspheres

โœ Scribed by Joseph Mirro Jr.; Jill F. Schwartz; Curt I. Civin


Book ID
118898381
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
611 KB
Volume
47
Category
Article
ISSN
0022-1759

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We describe a method for the ultrastructural localization of surface markers on cells in intact tissue sections. Using a primary monoclonal antibody to a surface marker followed by a second stage gold-labelled antibody, surface antigens can be specifically localized. This enables cellular interactio