✦ LIBER ✦
Electron microscopic localization of cell-surface markers in tissue sections using monoclonal and gold-conjugated antibodies
✍ Scribed by N. Rooney; C. Day; T. Gray; J. C. E. Underwood
- Book ID
- 104505605
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 598 KB
- Volume
- 148
- Category
- Article
- ISSN
- 0022-3417
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✦ Synopsis
We describe a method for the ultrastructural localization of surface markers on cells in intact tissue sections. Using a primary monoclonal antibody to a surface marker followed by a second stage gold-labelled antibody, surface antigens can be specifically localized. This enables cellular interactions to be studied in solid tissues.