Simultaneous analysis of c-erb B-2 expression and DNA content in breast cancer using flow cytometry
✍ Scribed by Olle Stål; Siw Sullivan; Xiao-Feng Sun; Sten Wingren; Bo Nordenskjöld
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 884 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0196-4763
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Background. Amplification of the c-erbB-2 protooncogene and DNA aneuploidy have been reported to correlate with poor patient prognosis in human breast cancer. Several studies have investigated the prognostic value of these two factors in heterogeneous populations of patients with node-positive and n
A PCR assay using capillary electrophoresis was designed for the detection of c-erbB-2 gene amplification in alcoholformalin-acetic acid (AFA)-fixed, paraffin-embedded biopsies from 81 consecutive breast tumors. c-erbB-2 expression was analyzed in the same samples using immuno-histochemistry (IHC).
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