Chromosome 1 aneusomy with 1p36 under-representation is related to histologic grade, DNA aneuploidy, high c-erb B-2 and loss of bcl-2 expression in ductal breast carcinoma
✍ Scribed by Fulvia Farabegoli; Claudio Ceccarelli; Donatella Santini; Davide Trerè; Nicola Baldini; Mario Taffurelli; Massimo Derenzini
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- French
- Weight
- 870 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Chromosome I abnormalities with loss of lp36 have been investigated in 95 breast-cancer samples by means of a dualtarget fluorescence in-sku hybridization (FISH) technique using the pUC I .77 and p 1-79 probes, specific for the I q I 2 and I p36 regions, respectively. Abnormalities for one or both probes were detected in 83/95 samples. Relative I p36 under-representation was found in 79/95. The clinical relevance of these alterations was studied by comparing the FISH results with several parameters currently used in breast-cancer pathology. Distinct patterns of chromosome I abnormalities were found among the histologic types of breast carcinoma. Lobular or mucinous samples showed few or no alterations, whereas most ductal samples had high chromosome I polysomy with underrepresentation of I p36. In ductal carcinomas, chromosome I alterations increased with histologic grade, DNA aneuploidy, loss of bcl-2 and high c-erb B-2 expression. These associations were found to be statistically significant. No correlation between chromosome I alterations and nuclear grade, age, size, lymph-node involvement, hormonal receptor presence, proliferation activity or p53 protein expression was detected. These results indicate the utility of this FISH technique for a better definition of the biological characteristics of ductal carcinomas.