Simultaneous Acquisition of 15N- and 13C-Edited NOE Spectra of Proteins Dissolved in H2O
โ Scribed by S.M. Pascal; D.R. Muhandiram; T. Yamazaki; J.D. Formankay; L.E. Kay
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 390 KB
- Volume
- 103
- Category
- Article
- ISSN
- 1064-1866
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โฆ Synopsis
We thank Alex Singer for preparing the PLC ({\gamma 1}) SH2 ({2}) sample used in this study and Steve Shoelson. Harvard University, for the generous gift of the pY 1021 phosphopeptide. This work was supported through grants from the Natural Sciences and Engineering Research Council of Canada (L.E.K.) and the National Cancer Institute of Canada (J.D.F. and L.E.K.) with funds from the Canadian Cancer Society. S. M. Pascal and Toshio Yamazaki are the recipients of postdoctoral fellowships from the Medical Research Council of Canada and the Human Frontiers Science Program, respectively.
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The precision of a protein structure determined by NMR spectroscopy is strongly influenced by the number of longrange NOE interactions per residue. Aromatic and methylcontaining residues are frequently located in the hydrophobic interior of proteins and yield long-range NOE interactions. Methyl grou
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