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Simulation of penicillin production in fed-batch cultivations using a morphologically structured model

✍ Scribed by Teresa C. Zangirolami; Claus L. Johansen; Jens Nielsen; Sten Bay Jørgensen

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
316 KB
Volume
56
Category
Article
ISSN
0006-3592

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✦ Synopsis

A mathematical model is formulated to describe trends in biomass and penicillin formation as well as substrate consumption for fed-batch cultivations. The biomass is structured into three morphological compartments, and glucose and corn steep liquor are considered as substrates for growth. Penicillin formation is assumed to take place in the subapical compartment and in the growing region of the hyphal compartment. Furthermore, it is inhibited by glucose. Model parameters are estimated using an evolutionary algorithm and fitting the model to a standard fed-batch cultivation. The model is validated on experimental data from three different fedbatch cultivations, including two repeated fed-batch cultivations. The model predictions show good agreement with the measurements of biomass and pencillin concentrations for all fed-batch cultivations.

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