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Simplified test for detecting the resistance of herpes simplex virus to acyclovir

✍ Scribed by Henri Agut; Jean-Thierry Aubin; Didier Ingrand; Sylvie Blanc; Anne-Louise Clayton; Shireen M. Chantler; Jean-Marie Huraux

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
631 KB
Volume
31
Category
Article
ISSN
0146-6615

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The detection of herpes simplex virus (HSV) antigen by means of an enzyme amplified ELISA was investigated for rapid screening of acyclovir (ACV) resistance. Vero cell monolayers were inoculated with various strains of HSV and incubated in the presence of different concentrations of ACV. When cytopathic effect was present, the culture supernatants were tested by ELISA. The absorbance values were found to correlate with the results of virus yield and plaque reduction assays. The comparison between absorbance values obtained in the presence of 10 μM ACV and in the absence of drug provided the basis for a simplified sensitivity test. The use of a single ACV concentration allowed discrimination between ACV‐resistant and ACV‐sensitive reference strains, the detection of ACV‐resistant virus mixed in the proportion of 10% with ACV‐sensitive virus, and a study of the emergence of an ACV‐resistant virus population in serial samples taken from experimental rabbit keratitis. The simplified susceptibility assay is a sensitive and convenient method for rapid screening of HSV resistance to ACV.

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