A novel multiplex nested polymerase chain reaction (PCR) assay was designed and evaluated for routine diagnosis of herpes simplex virus (HSV) infections in patients with either putative HSV infection of the central nervous system or suspected HSV keratitis. Single-tube amplification of HSV type 1 (H
A simple and rapid test for the identification of clinical herpes simplex virus isolates
โ Scribed by Gerald J. Lancz; Steven C. Specter
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- English
- Weight
- 315 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0146-6615
No coin nor oath required. For personal study only.
โฆ Synopsis
Abstract
A simple procedure is described which permits the rapid identification of clinical herpes simplex virus isolates. The test utilizes Staphylococcus aureus to which antiviral immunoglobulins had been adsorbed. Adherence of the antibodyโcoated bacteria to virusโinfected cells is readily seen by light microscopy. Indirect immunofluorescence and the S aureus adherence reaction were found to be approximately of equal sensitivity for the detection of virus antigens.
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