Simplified technique for differential staining of inner cell mass and trophectoderm cells of mouse and bovine blastocysts
β Scribed by G.A. Thouas; N.A. Korfiatis; A.J. French; G.M. Jones; A.O. Trounson
- Book ID
- 119611253
- Publisher
- Reproductive Healthcare Ltd
- Year
- 2001
- Tongue
- English
- Weight
- 306 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1472-6491
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## Abstract The two cell populations of the day 5 mouse blastocystβthe inner cell mass (ICM) and trophectoderm (TE)βwere isolated by immunosurgery and TE biopsy. The uptake of glucose and pyruvate and the production of lactate were measured in the isolated cell populations and in single day 5 blast
## Abstract The proportion of total cells in the blastocyst allocated to the inner cell mass (ICM) and trophectoderm (TE) is important for future development and may be a sensitive indicator to evaluate culture conditions. The number of cells and their distribution within the two primary cell linea