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P-224: Evaluation of different methods of cell fixation and cell membrane permeabilization for the differential staining of inner cell mass (ICM) and trophectoderm cells (TE) of mouse blastocysts

✍ Scribed by A.S. Santos-Bredariol; D.A. Januário; M. Maluf; C.E. Czeresnia; P.H. Saldiva; P.M. Perin

Book ID
116469851
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
87 KB
Volume
86
Category
Article
ISSN
1556-5653

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✍ Handyside, Alan H. ;Hunter, Susan 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 484 KB 👁 1 views

A rapid procedure has been devised to count the numbers of outer trophectoderm (TE) and inner cell mass (ICM) cells of mouse blastocysts by differentially labelling their nuclei in situ with polynucleotide-specific fluorochromes. The TE nuclei were labelled with propidium iodide (PI) by permeabilisi