Simplified solid-phase immunobead assay for detection of ciguatoxin and related polyethers

✍ Scribed by Yoshitsugi Hokama

• Book ID: 102307372
• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 382 KB
• Volume: 4
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860040313

✦ Synopsis

This study examined the development of a highly simplified solid-phase colored latex imrnunobead assay for the detection of ciguatoxin and related polyethers. This procedure was compared with the stick enzyme immunoassay previously reported. Chi-square analysis of two separate experiments on 153 and 283 fish of various species gave x2 values of p < 0.001 and p < 0.005, respectively Agree-ment between the two procedures with 26 fish implicated in ciguatera poisoning was 100°/o. A preliminary assessment in the field showed encouraging results. The procedure appears to be simple and applicable to field use. Furthermore, this procedure should be applicable to other antibody-antigen detections, especially low dalton determinations.