Simplified production and concentration of lentiviral vectors to achieve high transduction in primary human T cells

Systematic analysis of clinically applic

Systematic analysis of clinically applicable conditions leading to a high efficiency of transduction and transgene expression in human T cells

✍ María L. Lamana; José C. Segovia; Guillermo Guenechea; Juan A. Bueren 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 256 KB 👁 2 views

Background The transduction of human peripheral blood T cells with retroviral vectors constitutes an attractive approach for the correction of a number of genetic diseases. In this study we have conducted a systematic analysis of the relevance of a large number of parameters currently considered to