Systematic analysis of clinically applicable conditions leading to a high efficiency of transduction and transgene expression in human T cells
✍ Scribed by María L. Lamana; José C. Segovia; Guillermo Guenechea; Juan A. Bueren
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 256 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1099-498X
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✦ Synopsis
Background The transduction of human peripheral blood T cells with retroviral vectors constitutes an attractive approach for the correction of a number of genetic diseases. In this study we have conducted a systematic analysis of the relevance of a large number of parameters currently considered to affect the transduction of, and transgene expression in, human T cells.
Methods Retroviral vectors encoding the human nerve growth factor receptor (NGFR) were used for transducing human T cells from normal volunteers. The proportion of T cells that expressed the marker transgene was determined by ¯ow cytometry using anti-NGFR antibodies.
Results Spinoculation and static ®bronectin (FN)-assisted infections improved to a similar extent the transduction ef®ciency of PHA/IL-2 stimulated T cells, when compared with samples subjected to standard static infections. When immobilized anti-CD3 (anti-CD3i) or anti-CD3i/28istimulated T cells were considered, static infections in FN-coated plates were reproducibly more ef®cient than spinoculation infections performed on FNuncoated plates. Under optimized manipulation conditions (three infection cycles of anti-CD3i/28i-stimulated T cells in FN-coated plates) the total number of NGFR + T cells harvested after 7 days of incubation represented, on average, twice the total number of T cells seeded at Day 0, and up to 95% of the human T cells ef®ciently expressed the marker transgene. Similar results were obtained regardless of whether samples were manipulated in medium supplemented with fetal bovine serum or with heat-inactivated autologous serum.
Conclusions Our study offers new experimental conditions for the transduction of human T cells, with obvious implications for the development of gene therapy protocols.