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Simple single-step single-enzyme synthesis of [14C]-GSH

โœ Scribed by Steve A. de Keczer; Tatyana Voronin; Jennifer Yao; Fang-Jie Zhang; Mohammad R. Masjedizadeh

Publisher
John Wiley and Sons
Year
2010
Tongue
French
Weight
110 KB
Volume
53
Category
Article
ISSN
0022-2135

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โœฆ Synopsis

Abstract

The triโ€peptide [^14^C]โ€glutathione ([^14^C]โ€GSH) was synthesized in a single step by GSH synthetase catalyzed reaction of Lโ€ฮณโ€glutamylโ€Lโ€cysteine and [^14^C]โ€glycine. Preparative reverse phase HPLC afforded [^14^C]โ€GSH in 30% yield and 98% purity. Preparation of GSH synthetase from E. coli via recombinant DNA and the interconversion of [^14^C]โ€GSH to the disulfide [^14^C]โ€GSSG for storage are discussed. Copyright ยฉ 2010 John Wiley & Sons, Ltd.

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