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Simple measurement of gluconeogenesis by direct 2H NMR analysis of menthol glucuronide enrichment from 2H2O

✍ Scribed by Angela Ribeiro; M. Madalena Caldeira; Manuela Carvalheiro; Margarida Bastos; Carla Baptista; Ana Fagulha; Luisa Barros; Cristina Barosa; John G. Jones

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
232 KB
Volume
54
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

The contribution of gluconeogenesis to fasting glucose production was determined by a simple measurement of urinary menthol glucuronide (MG) ^2^H enrichment from ^2^H~2~O. Following ingestion of ^2^H~2~O (0.5% body water) during an overnight fast and a pharmacological dose (400 mg) of a commercial peppermint oil preparation the next morning, 364 μmol MG was quantitatively recovered from a 2‐h urine collection by ether extraction and a 125 μmol portion was directly analyzed by ^2^H NMR. The glucuronide ^2^H‐signals were fully resolved and their relative intensities matched those of the monoacetone glucose derivative. The pharmacokinetics and yields of urinary MG after ingestion of 400 mg peppermint oil as either gelatin or enteric‐coated capsules 1 h before breakfast were quantified in five healthy subjects. Gelatin capsules yielded 197 ± 81 μmol of MG from the initial 2‐h urine collection while enteric‐coated capsules gave 238 ± 84 μmol MG from the 2‐ to 4‐h urine collection. Magn Reson Med 54:429–434, 2005. © 2005 Wiley‐Liss, Inc.

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