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Simple Isolation Method and Assay for T4 DNA Ligase and Characterization of the Purified Enzyme

✍ Scribed by Karl-Werner KNOPF


Book ID
115115853
Publisher
John Wiley and Sons
Year
1977
Tongue
English
Weight
590 KB
Volume
73
Category
Article
ISSN
1432-1327

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A simple method for the isolation and ch
✍ Brendel, Martin πŸ“‚ Article πŸ“… 1976 πŸ› Springer 🌐 English βš– 586 KB

The mutant tmpl--10ts which confers thermosensitive auxotrophy for thymidylate is employed for the selection of 5'-dTMP uptaking mutants. At the nonpermissive temperature yeast cells phenotypically wild type for thymidylate uptake can grow for only 3 to 4 generations in the presence of 10(-2) M 5'-d