Using sucrose gradient centrifugation, anion exchange chromatography on a SMART system, isoelectric focusing (IEF) and sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS PAGE), a 45.5 kDa membrane protein was isolated from amoebae of the cellular slime mould Dictyostelium discoideum. The
✦ LIBER ✦
A rapid, simple method for the isolation and characterization of the photoreceptor of Dictyostelium discoideum amoebae
✍ Scribed by Thomas Schlenkrich; Markus Porst; Donat-P. Häder
- Book ID
- 115930763
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 292 KB
- Volume
- 364
- Category
- Article
- ISSN
- 0014-5793
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## Abstract Two different methods for the localization of Con A indicate that this lectin is bound to the surface of __Dictyostelium discoideum__ amoebae. Redistribution of the Con A receptors occurs both on cells in suspension and cells moving on an agar surface. Internalization of the Con A does
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