Simple graphical methods for use with complex ligand-binding and enzyme mechanisms
โ Scribed by Crabbe M.J.C
- Book ID
- 115920699
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 57 KB
- Volume
- 239
- Category
- Article
- ISSN
- 0014-5793
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๐ SIMILAR VOLUMES
BASIC computer programs have been designed to calculate enzyme kinetic and ligand binding parameters using distribution-free methods. Statistical estimates for the parameters can be calculated, and models distinguished by the F-test. Integrated rate equations are derived for complex enzyme mechanism
The theory and practice of a simple fluorimetric method for the estimation of ligand binding parameters in ligand-enzyme complexes is described. In this method, the concentration of a ligand-binding enzyme and the dissociation constant of a ligand-enzyme complex were estimated solely from the total
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