𝔖 Bobbio Scriptorium
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Simple gel electrophoresis procedure for purification of Achromobacter fischeri nitrite reductase

✍ Scribed by Mazhar Husain; J.C. Sadana

Book ID
102982018
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
708 KB
Volume
45
Category
Article
ISSN
0003-2697

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✦ Synopsis

were determined by the nitrosonaphthol reaction of Udenfriend, Weissbach, and Clark (13,14), Table 1 shows the recovery rate for 5HTP, 5HTPA, and 5HIA over the range of 30-600 nmoles. The mean per cent recovery was: for 5HTP, 98.0%, with a range of 95-100.3%; for 5HTPA, 97.1%, with a range of 96.2-99.4s; and for 5HIA, 97.6%, with a range of 97.5-100.2oJo.

This method is reproducible and useful for the determination of 5HTP, 5HTPA, and 5HIA present together in biological fluids.

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A simple and inexpensive procedure for preparative polyacrylamide gel electrophoresis of RNA
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## A method that is both simple and inexpensive is described for preparative polyacrylamide gel electrophoresis of low-molecular-weight RNA. The utility of the method is demonstrated by the preparative separation of 4S and 5s RX-i.