Simple freezing procedure for storage in serum-free media of cultured and tumor cells of mouse
β Scribed by Charity Waymouth; Don S. Varnum
- Publisher
- Springer
- Year
- 1976
- Tongue
- English
- Weight
- 226 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1573-0603
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A comparison was made of growth and drug-response of five human tumor cell lines (HT-29, colon carcinoma; TWI, melanoma; A-549, lung carcinoma; Panc-1 , pancreatic carcinoma; and El, bladder carcinoma) in serum-free media (SFM) and in serum-supplemented media (SSM) using the human tumor-clonogenic a
## Abstract Summary: C57BL/6 is a wellβcharacterized mouse strain that is used extensively for immunological and neurological research. The establishment of C57BL/6 ES cell lines has facilitated the study of geneβaltered mice in a pure genetic backgroundβhowever, relatively few such lines exist. Us
The procedure of analyzing hormone and growth factor requirements for the growth of MPC-I I cells and of developing a serum-free medium for this cell line has been described. In this medium, MPC-I I cells grow as fast as in serum-supplemented medium, up to 50 generations. MPC-I I cells grown in ser