Cotyledons from germinating seeds of the soybean cultivar Peking were inoculated with virulent Agrobacterium tumefaciens strain A281:pZA-7 which carries a wild type Ti plasmid pTiBo542 and a disarmed Ti plasmid (a binary vector)pZA-7 which contains the Ξ²glucuronidase (uidA) and neomycin phosphotrans
Simple estimate of ureides in soybean tissue
β Scribed by T.G. Patterson; R. Glenister; T.A. LaRue
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 411 KB
- Volume
- 119
- Category
- Article
- ISSN
- 0003-2697
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β¦ Synopsis
Extracts of soybean tissue are treated with an acidic cation-exchange resin to remove amino acids. The sample is treated with hypochlorite, pH 4.0, to convert the amide from allantoic acid or allantoin to products that react with alkaline phenol to form indophenol. The procedure is inexpensive, sensitive, and rapid. The method may be adapted for automated analysis with the Technicon autoanalyzer.
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