A simple procedure for the expression of genes in transgenic soybean callus tissue

✍ Scribed by G. Luo; A. Hepburn; J. Widholm

• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 787 KB
• Volume: 13
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00232935

✦ Synopsis

Cotyledons from germinating seeds of the soybean cultivar Peking were inoculated with virulent Agrobacterium tumefaciens strain A281:pZA-7 which carries a wild type Ti plasmid pTiBo542 and a disarmed Ti plasmid (a binary vector)pZA-7 which contains the βglucuronidase (uidA) and neomycin phosphotransferase (nptII) genes. Tumors were produced on all inoculated explants and 82% of these tumor lines were cotransformed by the nptII gene from the binary vector pZA-7 as shown by PCR analysis (18 of 22 lines tested). Eleven of these 18 lines were also resistant to kanamycin. Eleven lines expressed β-glucuronidase activity (GUS), six of which were also kanamycin resistant. Since there is a high rate of coexpression of genes carried by the binary vector, this system provides a simple and rapid method for the expression of genes of interest in transformed soybean tissue which has been used successfully to test constructs designed for soybean transformation.