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Cell type specific expression of a CaMV 35S-GUS gene in transgenic soybean plants

✍ Scribed by Yang, Ning-Sun ;Christou, Paul

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
635 KB
Volume
11
Category
Article
ISSN
0192-253X

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✦ Synopsis

A number of independently derived transgenic soybean plants expressing a chimeric p-glucuronidase (GUS) gene under the control of the 35s CaMV promoter and a nopaline synthase polyadenylation signal were recovered using direct DNA transfer via electric discharge particle acceleration. Expression of GUS in R, plants was localized using thin tissue sections.

Many tissue types expressed GUS at various levels.

Pericycle cells in root, parenchyma cells in xylem, and phloem tissues of stem and leaf had high levels of enzyme activity. Procambium, phloem, and cortex cells in root, vascular cambium cells in stem, and the majority of cortex cells in leaf midrib, expressed low or no GUS activity. Intermediate levels of GUS activity were detected in leaf mesophyll cells, certain ground tissue cells in stem and leaf midrib, and in trichome and epidermal guard cells. Thus, we conclude that the 35s CaMV promoter is cell-type specific and is developmentally regulated in soybean.

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