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Simple determination of cysteine and cystine in dilute solutions by high-performance liquid chromatography with electrochemical detection using a DC-TAST polarograph

✍ Scribed by Danuta Sybilska; Marek Przasnyski; Barbara Mysior; Krystyna Samochocka


Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
345 KB
Volume
283
Category
Article
ISSN
1873-3778

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✦ Synopsis


The biologically very important cysteine (RSH))cystine (RSSR) system has been the subject of many investigations, including polarographic studies and amperometric titrations'J. The very simple polarographic method permits the simultaneous determination of both compounds; at positive potentials of the dropping mercury electrode, RSH undergoes the following anodic reaction:


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## Abstract Epinine was detected with a simple and disposable carbon fiber flow electrode after being separated by high‐performance liquid chromatography (HPLC). An alumina adsorption procedure was used for the extraction of epinine from urine samples. The extraction recoveries at 1, 3, and 6 ΞΌg/mL