𝔖 Bobbio Scriptorium
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Simple and rapid preparation of gapped plasmid DNA for incorporation of oligomers containing specific DNA lesions

✍ Scribed by Huixian Wang; John B. Hays

Book ID
111640209
Publisher
Humana Press Inc
Year
2001
Tongue
English
Weight
128 KB
Volume
19
Category
Article
ISSN
1073-6085

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A rapid and easy method for the isolation of plasmid DNA, both in analytical and preparative scale, is described. Using dye-buoyant density-gradient centrifugation in a vertical rotor, separation of covalentlv closed. sunercoiled plasmid DNA from relaxed circular and linear DNA is completed within 1