Silver staining of the centromeric area of acrocentric and Robertsonian metacentric mouse chromosomes

✍ Scribed by A. Rocchi; G. Prantera; M. Castro; E. Capanna

• Book ID: 104629720
• Publisher: Springer Netherlands
• Year: 1982
• Tongue: English
• Weight: 777 KB
• Volume: 60
• Category: Article
• ISSN: 0016-6707
• DOI: 10.1007/bf00121459

✦ Synopsis

A silver stain (Kt) technique was used to analyze the centromeric area in metacentric chromosomes originating from Robertsonian rearrangements in the mouse. The 2n = 40 all-acrocentric mouse karyotype and two Robertsonian-rearranged karyotypes (2n = 24 and 2n = 26 from Upper Valtellina) were used. The existence was demonstrated of a single centromeric pattern common to metacentric and to acrocentric chromosomes except for the Y, and consisting of two deeply stained dots, one per chromatid. In many cells this technique stains the nucleolar organizers and resolves the paracentromeric constitutive heterochromatin in chromomeres.