Silver staining of proteins in polyacrylamide gels
β Scribed by Chevallet, Mireille; Luche, Sylvie; Rabilloud, Thierry
- Book ID
- 109945915
- Publisher
- Nature Publishing Group
- Year
- 2006
- Tongue
- English
- Weight
- 287 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1750-2799
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A combined Coomassie blue-silver stain method has been developed in sodium dodecyl sulfatepolyacrylamide gels for the detection of proteins using the model compounds bovine serum albumin, lysozyme, and recombinant DNA-derived human insulin. Sensitivity was enhanced 2.2 to 8.6 times by the new method
Variations of the ultrasensitive silver staining method of B. R. Oakley, D. R. Kirsch, and N. R. Morris (Anal. Biochem. 105, 361-363 (1980)) have been tested. It was established that the reliability of the method was greatly improved if (i) free silver was carefully washed out before reduction with