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Requirement for cysteine in the color silver staining of proteins in polyacrylamide gels

✍ Scribed by Paul J. Chuba; Sunil Palchaudhuri

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
562 KB
Volume
156
Category
Article
ISSN
0003-2697

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πŸ“œ SIMILAR VOLUMES

A simplified ultrasensitive silver stain
A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels
✍ Berl R. Oakley; Donald R. Kirsch; N.Ronald Morris πŸ“‚ Article πŸ“… 1980 πŸ› Elsevier Science 🌐 English βš– 581 KB

We have simplified the highly sensitive silver stain of R. C. Switzer III, C. R. Merril, and S. Shifrin (1979, Anal. Biochem. 98, 231–237) for visualizing proteins in polyacrylamide gels. We have reduced the number of steps in the procedure from 10 to 6, simplified the reagents in each step, and red

Experimentally improved reliability of u
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✍ Margret Eschenbruch; Robert R. BΓΌrk πŸ“‚ Article πŸ“… 1982 πŸ› Elsevier Science 🌐 English βš– 785 KB

Variations of the ultrasensitive silver staining method of B. R. Oakley, D. R. Kirsch, and N. R. Morris (Anal. Biochem. 105, 361-363 (1980)) have been tested. It was established that the reliability of the method was greatly improved if (i) free silver was carefully washed out before reduction with