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Silencing SARS-CoV Spike protein expression in cultured cells by RNA interference

✍ Scribed by Yuanjiang Zhang; Tieshi Li; Ling Fu; Changming Yu; Yinghua Li; Xialian Xu; Yinyin Wang; Hongxiu Ning; Shuping Zhang; Wei Chen; Lorne A Babiuk; Zhijie Chang

Book ID: 118519345
Publisher: Elsevier Science
Year: 2004
Tongue: English
Weight: 318 KB
Volume: 560
Category: Article
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(04)00087-0

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## Abstract RNA interference (RNAi) is a potent mechanism for local silencing of gene expression and can be used to study loss‐of‐function phenotypes in mammalian cells. We used RNAi to knockdown specifically the expression of choline acetyltransferase (ChAT), the enzyme of acetylcholine biosynthes