𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Significance of promoter hypermethylation of p16 gene for margin assessment in carcinoma tongue

✍ Scribed by Parul Sinha; S. Bahadur; Alok Thakar; Ajay Matta; Muzafar Macha; Ranju Ralhan; S. Datta Gupta

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
491 KB
Volume
31
Category
Article
ISSN
1043-3074

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Background

Loss of p16 expression by promoter hypermethylation has been reported as an early event in the development of oral cancer. The aim of our study was to explore the prognostic implications of presence of promoter hypermethylation of p16 gene in surgical margins in carcinoma tongue.

Methods

A prospective analysis of 38 patients with resectable carcinoma tongue was carried out. DNA from tumor and the surgical margins was assessed by methylation‐specific polymerase chain reaction. Follow‐up duration was 17 to 37 months.

Results

About 86.8% of tumors showed promoter hypermethylation of p16 gene. Out of 30 patients with histologically free margins, 43.3% showed positivity on molecular assessment. Patients with positive molecular margins had a 6.3‐fold increased risk of having local recurrence as compared to patients with negative margins.

Conclusion

Promoter hypermethylation of p16 gene may serve as a useful molecular marker for predicting local recurrence in carcinoma tongue. © 2009 Wiley Periodicals, Inc. Head Neck, 2009

📜 SIMILAR VOLUMES

Assessment of gene promoter hypermethyla
Assessment of gene promoter hypermethylation for detection of cervical neoplasia
✍ G. Bea A. Wisman; Esther R. Nijhuis; Mohammad O. Hoque; Nathalie Reesink-Peters; 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 French ⚖ 134 KB

## Abstract Current cervical cancer screening is based on morphological assessment of Pap smears and associated with significant false negative and false positive results. Previously, we have shown that detection of hypermethylated genes in cervical scrapings using quantitative methylation‐specific

Promoter hypermethylation profile of tum
Promoter hypermethylation profile of tumor-associated genes p16, p15, hMLH1, MGMT and E-cadherin in oral squamous cell carcinoma
✍ Muthusamy Viswanathan; Nobuo Tsuchida; Govindaswamy Shanmugam 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 French ⚖ 211 KB

## Abstract Aberrant promoter hypermethylation of tumor‐associated genes leading to their inactivation is a common event in many cancer types. Using a sensitive restriction‐multiplex PCR method, we studied the promoter hypermethylation profile of the __p16, p15, hMLH1, MGMT__ and __E‐cad__ genes in

Hypermethylation of the APC (adenomatous
Hypermethylation of the APC (adenomatous Polyposis Coli) gene promoter region in human colorectal carcinoma
✍ Mikko O. Hiltunen; Leena Alhonen; Jari Koistinaho; Sanna Myöhänen; Matti Pääkkön 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French ⚖ 206 KB 👁 2 views

Germline mutations of the putative tumor suppressor gene APC are associated in high frequency with the familial adenomatous polyposis, predisposing the patients to colorectal neoplasia. Similarly, sequence analyses have revealed that in more than half of patients with sporadic colorectal carcinoma o

Promoter hypermethylation of fibulin 1 g
Promoter hypermethylation of fibulin 1 gene is associated with tumor progression in hepatocellular carcinoma
✍ Mitsuro Kanda; Shuji Nomoto; Yukiyasu Okamura; Masamichi Hayashi; Mitsuhiro Hish 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 430 KB

## Abstract Hepatocellular carcinoma (HCC) is one of the most common cancers world‐wide but the molecular mechanisms that underlie hepatocarcinogenesis are not fully determined. On the same surgical sample with HCC, we performed microarray‐based gene expression profiling and karyotype analysis usin

High frequency of promoter hypermethylat
High frequency of promoter hypermethylation of RASSF1A and p16 and its relationship to aflatoxin B1–DNA adduct levels in human hepatocellular carcinoma
✍ Yu-Jing Zhang; Habibul Ahsan; Yu Chen; Ruth M. Lunn; Li-Yu Wang; Shu-Yuan Chen; 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 154 KB

## Abstract Epigenetic changes in gene expression due to extensive CpG island methylation is now accepted as the main cause of inactivation of the __p16__ gene. More recently, it has been suggested that the human ras association domain family (RASSF) 1 gene, cloned from the lung tumor‐suppressor lo