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Hypermethylation of the APC (adenomatous Polyposis Coli) gene promoter region in human colorectal carcinoma

✍ Scribed by Mikko O. Hiltunen; Leena Alhonen; Jari Koistinaho; Sanna Myöhänen; Matti Pääkkönen; Sinikka Marin; Veli-Matti Kosma; Juhani Jänne

Publisher
John Wiley and Sons
Year
1997
Tongue
French
Weight
206 KB
Volume
70
Category
Article
ISSN
0020-7136

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✦ Synopsis

Germline mutations of the putative tumor suppressor gene APC are associated in high frequency with the familial adenomatous polyposis, predisposing the patients to colorectal neoplasia. Similarly, sequence analyses have revealed that in more than half of patients with sporadic colorectal carcinoma or adenoma, the APC gene was mutated. By employing genomic sequencing, i.e., base-specific analysis of methylated cytosines, we show here that the promoter region of the APC gene is heavily methylated at CpG sites in patients with colorectal carcinoma in comparison with normal colonic mucosa and premalignant adenomas. Our results suggest that cytosine methylation of the regulatory sequences of the APC gene could be involved in the progression of human colorectal cancer.

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