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Sieving of double-stranded DNA during agarose gel electrophoresis

โœ Scribed by Dr. Philip Serwer


Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
586 KB
Volume
10
Category
Article
ISSN
0173-0835

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By use of agarose gel electrophoresis, the sieving of spherical particles in agarose gels has been quantitated and modeled for spheres with a radius (R) between 13.3 and 149 nm. For quantitation, the electrophoretic mobility has been determined as a function of agarose percentage (A). Because a prev

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The field free diffusion constant and the electric field dependence of the electrophoretic mobility and molecular orientation of DNA samples from 5 to 164 kilobase pairs in agarose gels from 0.5 to 2% have been measured by fluorescence recovery after photobleaching and birefringence. In conditions w