In a previous paper [(1976) Anal. Biochem. 74, 2821 we reported a procedure for the analysis of 25hydroxycholecalciferol (25OH-Da) in cow plasma or serum by high performance liquid chromatography. Since then, we obtained synthetic 25hydroxyergocalciferol (25OH-D& and found that it had the same reten
Shorter high-performance liquid chromatograpic method for the determination of 25-hydroxycholecalciferol in cow serum
β Scribed by Koshy, K. T.; Van der Slik, A. L.
- Book ID
- 126253496
- Publisher
- American Chemical Society
- Year
- 1980
- Tongue
- English
- Weight
- 237 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0021-8561
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A high-pressure liquid chromatographic (hplc) procedure was developed for the determination of 2%hydroxycholecalciferol(25-OH-DJ in cow plasma or serum. The procedure involved extraction with an ethanol-ethyl ether mixture, separation of the aqueous phase, solvent partitions, column chromatography o
A reversed-phase HPLC method for determination of 25-hydroxycholecalciferol (25-OH D3) in serum samples is described. The method involves extraction of 25-OH D, with hexane, followed by differential resolubilization in acetonitrile. The sample is then applied directly on the CI8 bonded phase column