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High-performance liquid chromatographic method for the determination of 25-hydroxycholecalciferol in chicken egg yolks

โœ Scribed by Koshy, K. Thomas; Van Der Slik, Allen L.

Book ID
127370769
Publisher
American Chemical Society
Year
1979
Tongue
English
Weight
534 KB
Volume
27
Category
Article
ISSN
0021-8561

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In a previous paper [(1976) Anal. Biochem. 74, 2821 we reported a procedure for the analysis of 25hydroxycholecalciferol (25OH-Da) in cow plasma or serum by high performance liquid chromatography. Since then, we obtained synthetic 25hydroxyergocalciferol (25OH-D& and found that it had the same reten

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A high-pressure liquid chromatographic (hplc) procedure was developed for the determination of 2%hydroxycholecalciferol(25-OH-DJ in cow plasma or serum. The procedure involved extraction with an ethanol-ethyl ether mixture, separation of the aqueous phase, solvent partitions, column chromatography o