Short Term Kinetics of RNA Labelling in HeLa Cells
β Scribed by D. SZAPARY-WINCKELMANS; M. BRUNFAUT; M. EERRERA
- Book ID
- 115111625
- Publisher
- John Wiley and Sons
- Year
- 1968
- Tongue
- English
- Weight
- 932 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1432-1327
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π SIMILAR VOLUMES
## Abstract Further evidence of mRNA in HeLa cells with a halfβlife two hours or less is given. A kinetic model of RNA synthesis in HeLa cells is described in which equilibration of label occurs first into the acid soluble pool (evidence is given that this pool feeds RNA synthesis) and thence in nu
%-Labeled Met-tRNArMet can be prepared from HeLa cells, for studies of translation in vitro, with both a high degree of charging and a relatively high specific radioactivity. HeLa cells are labeled with [%]methionine in vivo, in the presence of cycloheximide to reduce translation. Their cytoplasmic