✦ LIBER ✦
Highly charged radioactive initiator methionyl transfer RNA from in vivo labeled HeLa cells
✍ Scribed by G. Bolcsfoldi; E. Ritter; M.H. Vaughan Jr.
- Book ID
- 102629093
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 314 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
%-Labeled Met-tRNArMet can be prepared from HeLa cells, for studies of translation in vitro, with both a high degree of charging and a relatively high specific radioactivity. HeLa cells are labeled with [%]methionine in vivo, in the presence of cycloheximide to reduce translation. Their cytoplasmic RNA is then isolated by phenol extraction and subjected to cellulose ion-exchange chromatography in order to partially purify labeled Met-tRNArMet and resolve it from Met-tRNA,Met.